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Raman spectroscopic study of serum albumins: an effect of proton‐ and γ‐irradiation
Author(s) -
Synytsya Alla,
Alexa Petr,
de Boer Jorrit,
Loewe Markus,
Moosburger Martin,
Würkner Michael,
Volka Karel
Publication year - 2007
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1884
Subject(s) - raman spectroscopy , chemistry , bovine serum albumin , irradiation , denaturation (fissile materials) , proton , albumin , human serum albumin , amino acid residue , serum albumin , amino acid , biochemistry , nuclear chemistry , peptide sequence , physics , quantum mechanics , gene , nuclear physics , optics
Raman spectroscopy was applied to analyse structural changes in serum albumins (bovine serum albumin, BSA; human serum albumin, HSA) following proton and γ‐irradiation (0.5, 5 and 50 Gy). Characteristic Raman bands of both polypeptide backbone and amino acid residues were sensitive to irradiation. Significant damage of HSA/BSA was observed only at the highest dose (50 Gy). Raman spectra confirmed radiation‐induced denaturation, destruction of helical structures and aggregation of serum albumins. The differences in the dose‐dependent effects of proton and γ‐radiation on studied proteins are discussed. Copyright © 2007 John Wiley & Sons, Ltd.