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The investigation of single bacteria by means of fluorescence staining and Raman spectroscopy
Author(s) -
Krause M.,
Radt B.,
Rösch P.,
Popp J.
Publication year - 2007
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1721
Subject(s) - raman spectroscopy , dapi , fluorescence , bacteria , staining , labelling , chemistry , biophysics , biology , biochemistry , analytical chemistry (journal) , chromatography , optics , genetics , physics
The successful combination of Raman spectroscopy and fluorescence labelling of single bacterial cells is presented. The results show that the selected dyes (DAPI, SYTO 9, CFDA, SYTOX blue and PI) in most cases do not influence the Raman spectrum of a labelled bacterium significantly compared to an unstained bacterium spectrum. The labelling of bacteria offers the possibility of live/dead or biotic/abiotic differentiation prior to a Raman identification step. Copyright © 2007 John Wiley & Sons, Ltd.