Premium
Immuno‐Raman microspectroscopy: In situ detection of antigens in tissue specimens by surface‐enhanced Raman scattering
Author(s) -
Schlücker S.,
Küstner B.,
Punge A.,
Bonfig R.,
Marx A.,
Ströbel P.
Publication year - 2006
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1534
Subject(s) - raman spectroscopy , raman scattering , in situ , antigen , chemistry , raman microspectroscopy , microbiology and biotechnology , analytical chemistry (journal) , materials science , optics , chromatography , biology , immunology , physics , organic chemistry
We demonstrate the first realization of immuno‐Raman microspectroscopy, a novel methodology in immunohistochemistry. The in situ detection of antigens in tissue specimens is achieved by surface‐enhanced Raman scattering (SERS) from aromatic Raman labels covalently linked to the corresponding antibody. As an example of applications of this new Raman technique, we present the localization of prostate‐specific antigen (PSA) in epithelial tissue from patients with prostate carcinoma (PCA). Copyright © 2006 John Wiley & Sons, Ltd.