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In vivo time‐resolved Raman imaging of a spontaneous death process of a single budding yeast cell
Author(s) -
Naito Yasuaki,
Tohe Akio,
Hamaguchi Hiroo
Publication year - 2005
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1397
Subject(s) - budding , vacuole , raman spectroscopy , yeast , in vivo , programmed cell death , budding yeast , microbiology and biotechnology , saccharomyces cerevisiae , chemistry , cell , confocal , biophysics , biology , biochemistry , apoptosis , optics , cytoplasm , genetics , physics
In vivo confocal Raman microspectroscopy has been used to study a spontaneous death process of a single budding yeast ( S. cerevisiae ) cell. The observed time‐resolved Raman images indicate that, concomitantly with the sudden appearance of a dancing body in a vacuole, the mitochondrial metabolic activity of the cell is markedly lowered and that the subsequent disappearance of the vacuole is followed by an inevitable cell death within a few hours. Copyright © 2005 John Wiley & Sons, Ltd.