Premium
Surface‐enhanced Raman spectroscopy of bacteria: the effect of excitation wavelength and chemical modification of the colloidal milieu
Author(s) -
Zeiri L.,
Efrima S.
Publication year - 2005
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1349
Subject(s) - raman spectroscopy , chemistry , colloid , surface enhanced raman spectroscopy , excitation , spectroscopy , analytical chemistry (journal) , resonance raman spectroscopy , laser , nanotechnology , raman scattering , photochemistry , materials science , optics , organic chemistry , physics , electrical engineering , quantum mechanics , engineering
Surface‐enhanced Raman spectroscopy (SERS) of bacteria infused with silver or gold colloids reflects the different biochemicals in the immediate, nano‐vicinity of the particles. In particular we demonstrate high sensitivity to flavins, important coenzymes usually associated with the cell wall. Varying the type of colloid, the laser excitation wavelength and the chemical protocols enables us to observe spectral signatures from different biochemical components of the microorganism. Specifically, we can switch between normal Raman, resonance Raman, and SERS, altering the relative spectral contributions of the various cell constituents. Copyright © 2005 John Wiley & Sons, Ltd.