Raman spectroscopic identification of single yeast cells | Zendy

Rösch Petra | Zendy; Harz Michaela | Zendy; Schmitt Michael | Zendy; Popp Jürgen | Zendy

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Raman spectroscopic identification of single yeast cells

Author(s) -

Rösch Petra,

Harz Michaela,

Schmitt Michael,

Popp Jürgen

Publication year - 2005

Publication title -

journal of raman spectroscopy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.748

H-Index - 110

eISSN - 1097-4555

pISSN - 0377-0486

DOI - 10.1002/jrs.1312

Subject(s) - yeast , raman spectroscopy , identification (biology) , compartmentalization (fire protection) , cell , single cell analysis , nucleus , saccharomyces cerevisiae , biology , chemistry , computational biology , biochemistry , microbiology and biotechnology , biophysics , physics , botany , optics , enzyme

Eukaryotes (such as yeasts) and prokaryotes (such as bacteria) differ in size, molecular complexity, etc. By definition, eukaryotic cells store their DNA in a separate internal compartment, the so‐called nucleus. Owing to this molecular compartmentalization, the identification of yeast cells by vibrational spectroscopy at a single‐cell level is challenging. This contribution reports on first results of micro‐Raman analysis together with a hierarchical cluster analysis allowing for an online identification method for yeast cells at a single‐cell level. For the classification analysis, an average spectrum from 10 different measuring points within a single yeast cell was used to overcome the heterogeneity of the yeast cells. Copyright © 2005 John Wiley & Sons, Ltd.

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