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Raman spectroscopic identification of single yeast cells
Author(s) -
Rösch Petra,
Harz Michaela,
Schmitt Michael,
Popp Jürgen
Publication year - 2005
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1312
Subject(s) - yeast , raman spectroscopy , identification (biology) , compartmentalization (fire protection) , cell , single cell analysis , nucleus , saccharomyces cerevisiae , biology , chemistry , computational biology , biochemistry , microbiology and biotechnology , biophysics , physics , botany , optics , enzyme
Eukaryotes (such as yeasts) and prokaryotes (such as bacteria) differ in size, molecular complexity, etc. By definition, eukaryotic cells store their DNA in a separate internal compartment, the so‐called nucleus. Owing to this molecular compartmentalization, the identification of yeast cells by vibrational spectroscopy at a single‐cell level is challenging. This contribution reports on first results of micro‐Raman analysis together with a hierarchical cluster analysis allowing for an online identification method for yeast cells at a single‐cell level. For the classification analysis, an average spectrum from 10 different measuring points within a single yeast cell was used to overcome the heterogeneity of the yeast cells. Copyright © 2005 John Wiley & Sons, Ltd.