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1064 nm near‐infrared multichannel Raman spectroscopy of fresh human lung tissues
Author(s) -
Min YoungKun,
Yamamoto Tatsuya,
Kohda Ehiichi,
Ito Toshiaki,
Hamaguchi Hiroo
Publication year - 2005
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1280
Subject(s) - raman spectroscopy , infrared , human lung , materials science , spectroscopy , infrared spectroscopy , near infrared spectroscopy , chemistry , analytical chemistry (journal) , lung , nuclear magnetic resonance , optics , medicine , physics , chromatography , organic chemistry , quantum mechanics
Abstract Raman spectra of fresh human lung tissues without any treatment such as freezing and/or formalin fixation were successfully recorded on a near‐infrared multichannel Raman system developed in our laboratory. It was confirmed that 1064 nm excitation was a requisite for the Raman study of fresh lung tissues; excitation at 785 nm failed to detect any Raman bands because of an extremely high fluorescence background. The observed Raman spectra of lung tissues made a clear distinction between the normal and cancerous states. We also succeeded in obtaining high signal‐to‐noise ratio Raman spectra of coagulated blood and carbon matter engulfed in the lung by scavenger cells. These results demonstrate the feasibility of 1064 nm near‐infrared multichannel Raman spectroscopy for in vivo , non‐invasive and molecular‐level clinical diagnosis of diseases including cancers. Copyright © 2005 John Wiley & Sons, Ltd.