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Analysis of 5‐fluorouracil in saliva using surface‐enhanced Raman spectroscopy
Author(s) -
Farquharson Stuart,
Shende Chetan,
Inscore Frank E.,
Maksymiuk Paul,
Gift Alan
Publication year - 2005
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1277
Subject(s) - raman spectroscopy , surface enhanced raman spectroscopy , saliva , chemistry , detection limit , thiocyanate , fluorouracil , drug detection , molecule , spectroscopy , analytical chemistry (journal) , nuclear chemistry , chromatography , inorganic chemistry , chemotherapy , biochemistry , raman scattering , organic chemistry , optics , surgery , medicine , physics , quantum mechanics
The ability of surface‐enhanced Raman spectroscopy (SERS) to measure the chemotherapy drug 5‐fluorouracil in saliva is presented. A silver‐doped sol–gel provided SERS and also some chemical selectivity. 5‐Fluorouracil and physiological thiocyanate produced SERS, whereas large biochemicals, such as enzymes and proteins, did not, supporting the expectation that the larger molecules do not diffuse through the sol–gel to any appreciable extent. In addition, 5‐fluorouracil samples of 2 µg ml −1 were easily measured, and an estimated limit of detection of 150 ng ml −1 in 5 min should provide sufficient sensitivity to perform pharmacokinetic studies and to monitor and regulate patient dosage. This would fill a critical need for this highly used drug, since genetic‐based variations in its metabolism can range by as much as five‐fold from one patient to another. Copyright © 2004 John Wiley & Sons, Ltd.