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Intercalation‐induced structural change of DNA as studied by 1064 nm near‐infrared multichannel Raman spectroscopy
Author(s) -
Yuzaki Kenro,
Hamaguchi Hiroo
Publication year - 2004
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1276
Subject(s) - raman spectroscopy , intercalation (chemistry) , ethidium bromide , chemistry , analytical chemistry (journal) , excited state , fluorescence , spectroscopy , dna , aqueous solution , inorganic chemistry , optics , chromatography , biochemistry , physics , quantum mechanics , nuclear physics
Using 1064 nm‐excited dispersive multichannel Raman spectroscopy, we were able to measure the Raman spectra of the ethidium bromide (EB)–DNA complex in aqueous solution. Excitation at 1064 nm decreased the fluorescence due to EB to a considerable extent, so that high signal‐to‐noise ratio Raman spectra of the DNA–EB complex were obtained. The effect of the intercalation of EB into calf thymus DNA was investigated for various EB/DNA (base pair) molar ratios ranging from 1/30 to 1/3. The Raman spectral changes clearly indicated a structural transition of DNA from the B‐form to an A‐like form with increasing the EB concentration. Thus, the intercalation‐induced structural change of DNA was successfully traced by Raman spectroscopy. Copyright © 2004 John Wiley & Sons, Ltd.