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Application of fourier transform Raman spectroscopy to biomembranes: 1064 NM excited Raman spectra of hog kidney mitochondrial outer membranes
Author(s) -
Omura Yoko,
Muraishi Syuichi
Publication year - 1995
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250260113
Subject(s) - raman spectroscopy , chemistry , analytical chemistry (journal) , fourier transform infrared spectroscopy , phospholipid , excited state , infrared , membrane , fluorescence , chromatography , biochemistry , physics , quantum mechanics , nuclear physics , optics
Fourier transform Raman spectra with 1064 nm excitation were measured at ambient temperature on hog kidney mitochondrial outer membrane preparations of ultracentrifuged pellet and almost fluorescence‐free Raman spectra with satisfactory signal‐to‐noise ratios were obtained. Fourier transform IR spectra were also measured. The observed Raman and infrared wavenumbers were tentatively assigned to membrane proteins and phospholipids, respectively. CH stretching, phospholipid CC stretching and PO 2 − stretching vibrations are discussed. To examine the applicability of near‐infrared excited Fourier transform Raman spectroscopy to the study of the inhibitory effects of inorganic ions on membrane‐bound monoamine oxidase activity, measurements were also made on membrane preparations suspended in phosphate buffer (pH 7.0) at ambient temperature in the absence (control) and presence of NaCl, and studies were made of phospholipid CC stretching and PO 2 − stretching vibrations, which are relatively unaffected by H 2 O self‐absorption by near‐infrared radiation. It was found that the presence of NaCl decreased the intensity of the 1080 cm − 1 gauche form acyl chain CC stretching Raman band relative to those of the all‐ trans CC stretching Raman band at 1126 and 1068 cm − 1 . This result was different from that obtained with aqueous dispersions of phosphatidylcholine (PC), for which it has been reported previously that monovalent ions such as Na + do not affect the I trans /I gauche ratio.