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Fourier transform Raman study of acid—base interactions via guanosine N‐7 in solid complexes and in strongly acidic solutions
Author(s) -
Bertoluzza A.,
Fini G.,
Tosi M. R.,
Tugnoli V.
Publication year - 1994
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250250734
Subject(s) - guanosine , raman spectroscopy , chemistry , nucleoside , base (topology) , aqueous solution , nucleic acid , crystallography , hydrogen bond , molecule , stereochemistry , organic chemistry , physics , biochemistry , mathematical analysis , mathematics , optics
Among the nucleosides, guanosine shows an anomalous behaviour which has been related to various factors (conformation, H‐bonding, self‐association, etc.), which characterize this nucleoside in relation to the others. It also easily forms a gel, the structure of which is well known. This work concerns the Raman vibrational spectroscopic characterization of the reactivity of guanosine N‐7, which is one of the main factors responsible for the anomalous behaviour of guanosine. For this purpose, some of its acid–base solid complexes and strongly acidic aqueous solutions were studied. In particular, this study considers the two Raman bands at about 1480 and 1540 cm −1 , attributable to vibration modes with prevailing imidazolic character, N‐7 of free guanosine or bonded guanosine, respectively. In addition, the acid–base interaction via the H‐bond of N‐7 and the proton transfer in relation to the ΔpK a . of the interacting group were studied.