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Raman optical activity instrument for biochemical studies
Author(s) -
Hecht L.,
Barron L. D.,
Gargaro A. R.,
Wen Z. Q.,
Hug W.
Publication year - 1992
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250230706
Subject(s) - raman optical activity , raman spectroscopy , monochromator , optics , holography , polarization (electrochemistry) , chemistry , grating , radiation , optoelectronics , materials science , wavelength , physics
A simple and fully computer‐controlled backscattering incident circular polarization Raman optical activity (ICP ROA) instrument suitable for chioptic studies of biologically significant molecules in aqueous solution is described. It consists of a fast single‐stage monochromator equipped with a holographic edge filter, a highly efficient holographic difiaction grating and a thinned back illuminated thermoelectrically cooled charge‐coupled device detector as the key spectral elements. A longitudinal electro‐optic modulator is employed to switch between orthogonal circular polarization states in the incident laser radiation within a backscattering geometry. A thick Lyot depolarizer is used for the depolarization of the backscattered Raman radiation. High‐quality backscattering ICP ROA spectra of (1 S )‐( − )‐ trans ‐pinane, (1 R )‐( + )‐ trans ‐pinane, L ‐alanine, L ‐alanyl‐ L ‐alanine, lysozyme, D ‐glucose, α‐ D ‐cyclodextrin and 2′‐deoxycytidine are presented as typical examples of the excellent performance characteristics.