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Surface‐enhanced Raman spectroscopic study of amsacrine and amsacrine–DNA interactions
Author(s) -
Barton Thomas F.,
Cooney Ralph P.,
Denny William A.
Publication year - 1992
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250230605
Subject(s) - amsacrine , acridine , ligand (biochemistry) , chemistry , raman spectroscopy , intercalation (chemistry) , acridine derivatives , fluorescence , dna , photochemistry , base pair , raman scattering , crystallography , analytical chemistry (journal) , stereochemistry , inorganic chemistry , organic chemistry , biochemistry , optics , topoisomerase , receptor , physics
Surface‐enhanced Raman scattering (SERS) spectroscopy has been employed to study the interaction of intercalating ligands with DNA. SERS was used to obtain spectra of acridine, aminoacridine, the 9‐anilinoacridine amsacrine and related compounds at associated solution concentrations down to 1 μM. A major advantage of SERS was the near absence of fluorescence normally inherent in solution spectra of these compounds. SERS spectra of amsacrine–DNA complexes at various ligand/base pair ratios showed free ligand concentrations consistent with those calculated from the binding constant, and showed that the less strongly interacting acridine was largely unbound even at high ligand/base pair ratios.