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Non‐resonant background suppression in preresonance CARS spectra of flavin adenine dinucleotide: Demonstration of a background suppression technique using phase mismatching and comparison with the polarization‐sensitive CARS technique
Author(s) -
Scholten T. A. H. M.,
Lucassen G. W.,
Koelewijn E.,
De Mul F. F. M.,
Greve J.
Publication year - 1989
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250200806
Subject(s) - chemistry , wavelength , polarization (electrochemistry) , analytical chemistry (journal) , cuvette , spectral line , flavin adenine dinucleotide , excited state , depolarization , molecular physics , nuclear magnetic resonance , optics , atomic physics , physics , chromatography , biochemistry , cofactor , astronomy , enzyme , medicine , endocrinology
Polarization‐sensitive CARS spectra of a 5.7 × 10 −3 mol dm −3 flavin adenine dinucleotide (FAD) solution were recorded under preresonance conditions at a pump wavelength of 532 nm. The depolarization ratios of the vibrations are shown to be close to the depolarization ratio of the non‐resonant background. This results in a severe reduction of the vibration resonant signal (a factor of 700‐900) in the polarization CARS spectrum, and a poor improvement in the ratio of the resonant signal and the non‐resonant background (<10). In this context, a non‐resonant background suppression technique is discussed and demonstrated for 5.7 × 10 −3 and 1.4 × 10 −3 mol dm −3 FAD solutions excited at 532 nm; the non‐resonant susceptibility of the walls of the cuvette, which contains the FAD solution, is used to compensate the non‐resonant signal contribution of the solution. An improvement in the signal‐to‐noise ratio of ca. 50 is achieved at the cost of a factor of 30 in the resonant signal strength. Lorentzian‐shaped spectral bands are obtained, facilitating the determination of band position, width and intensity. Line shape parameters and depolarization ratios for FAD are extracted from the presented spectra by curve fitting. The signal strength and background suppression achieved with these techniques and the resonance CARS technique (at a pump wavelength of 480 nm) are compared and discussed.