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Raman spectra and binding of Cu II to purine nucleotides
Author(s) -
Carmona P.,
Huong P. V.,
Gredilla E.
Publication year - 1988
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250190504
Subject(s) - chemistry , guanine , nucleotide , guanosine , nucleobase , raman spectroscopy , polynucleotide , stereochemistry , ribose , guanosine monophosphate , protonation , crystallography , moiety , glycosidic bond , nucleic acid , dna , biochemistry , enzyme , organic chemistry , ion , physics , optics , gene
Raman spectra of adenosine 5′‐monophosphate, poly(rA), guanosine 5′‐monophosphate and their Cu II complexes have been obtained and analysed by referring to spectra of D‐, 15 N‐ and 13 C‐labelled nucleobases. The greatest spectral changes on attachment of Cu II to these nucleotides are associated with changes in the five‐membered ring and suggest that the metal binds preferentially to N‐7 of nucleobases. However, in the Cu II coordinated poly(rA) species, N‐1 binding sites are also present to some extent, as deduced from the comparison of the Raman spectrum of the Cu‐poly(rA) complex with that of the protonated polynucleotide. In the complexes of guanosine 5′‐monophosphate with Cu II , the guanine residue is located at the anti position with respect to the ribose moiety through the glycosidic bond. In all cases, strong changes are also recorded for characteristic bands of phosphate groups, indicating their participation as ligands surrounding Cu II .