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UV resonance Raman study of the DNA–β‐propiolactone interaction
Author(s) -
Jollès Béatrice,
Chinsky Laurent,
Laigle Alain
Publication year - 1988
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250190303
Subject(s) - guanine , raman spectroscopy , chemistry , dna , nucleic acid , resonance raman spectroscopy , molecule , resonance (particle physics) , hydrolysis , alkylation , photochemistry , biochemistry , organic chemistry , nucleotide , atomic physics , catalysis , physics , optics , gene
The interaction between DNA and a carcinogenic molecule, β‐propiolactone (BPL), has been followed by UV resonance Raman spectroscopy. The excitation wavelength, 257 nm, is such that the information obtained concerns the changes in the nucleic bases. At the same time as the spontaneous pH decrease caused by the hydrolysis of some BPL molecules, alkylation at N‐7 of guanine is shown to occur. More sites of interaction are found after the enzymatic hydrolysis of DNA modified by BPL at neutral pH. Guanine is shown to be modified at several sites: N‐7, N‐2 and N‐1. Alterations of adenine are also found, in N‐1 and after a reorganization of the structure concerning the N‐1 and N‐6 positions.