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Raman spectroscopic study of Bacillus subtilis extracellular levansucrase: Effect of iron (III) ions on the protein conformation
Author(s) -
Gróf Pál,
Aslanian Dimitrina,
Chambert Regis
Publication year - 1988
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250190212
Subject(s) - raman spectroscopy , levansucrase , bacillus subtilis , chemistry , extracellular , ion , crystallography , biochemistry , organic chemistry , bacteria , biology , physics , optics , genetics
Vibrational Raman spectroscopy has been used to study the conformation of the extracellular enzyme levansucrase. Secondary structure analysis by the method of Williams shows 51% β‐sheets, 18% total α‐helical structure (from 7 to 16%) and modifies the environment of the Trp and Phe residues and also more of the CC, CN, CS and CH 3 groups.