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Ultraviolet resonance Raman bands of guanosine and adenosine residues useful for the determination of nucleic acid conformation
Author(s) -
Nishimura Yoshifumi,
Tsuboi Masamichi,
Kubasek William L.,
Bajdor Krzysztof,
Peticolas Warner L.
Publication year - 1987
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250180314
Subject(s) - furanose , guanine , chemistry , nucleic acid , raman spectroscopy , guanosine , uracil , nucleotide , resonance (particle physics) , resonance raman spectroscopy , stereochemistry , adenosine , crystallography , guanosine monophosphate , ring (chemistry) , dna , biochemistry , organic chemistry , physics , particle physics , optics , gene
The ultraviolet resonance Raman (UVRR) bands of several guanine‐ and adenine‐containing mononucleotides were measured, and were compared with the classical and UVRR spectra of 9‐ethylguanine, which contains no furanose ring, in order to assess the effect of sugar conformation on the UVRR spectrum. For several guanine mononucleotides containing different furanose rings some differences were found in the Raman bands normally attributed to the purine vibrations. A similar effect exists with different adenosine mononucleotides. The fact that these vibrations are allowed in the rigorous resonance Raman effect indicates that they are coupled to the electronic transitions of the base. Differences which are seen between cyclic nucleotides, ribonucleotides and deoxyribonucleotides probably arise from differences in the conformation of the attached furanose rings. This provides a method for the identification of conformationally sensitive vibrations of purine rings in nucleic acids.