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A Raman spectroscopic discrimination between the sub‐gel phase and the GIII Gel phase of aqueous dipalmitoylphosphatidylcholine bilayers
Author(s) -
Wong P. T. T.,
Mushayakarara E.,
Mantsch H. H.
Publication year - 1985
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250160612
Subject(s) - dipalmitoylphosphatidylcholine , raman spectroscopy , triclinic crystal system , phase (matter) , chemistry , phase transition , analytical chemistry (journal) , crystallography , bilayer , aqueous solution , phospholipid , chromatography , membrane , organic chemistry , crystal structure , phosphatidylcholine , optics , biochemistry , physics , quantum mechanics
Raman spectra of aqueous dipalmitoylphosphatidylcholine (DPPC) bilayers have been measured as a function of temperature for samples incubated at 2 °C and for non‐incubated samples. The nature of the transition from the GII gel phase of the hydrated lipid into the sub‐gel phase on incubation is entirely different from that of the transition from the GII gel phase into the GIII gel phase of the non‐incubated lipid. The GIII gel phase has a monoclinic interchain packing, whereas the sub‐gel phase exhibits a triclinic interchain structure. In the sub‐gel phase the acyl chains are rigid and more ordered even than those in solid DPPC.