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Raman microspectroscopy of LiDNA fibres in ethanol and tert ‐butanol
Author(s) -
Mud J.,
Otto C.,
De Mul F. F. M.,
Greve J.
Publication year - 1985
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250160605
Subject(s) - raman spectroscopy , ethanol , chemistry , raman microspectroscopy , butanol , salt (chemistry) , n butanol , analytical chemistry (journal) , chromatography , nuclear chemistry , organic chemistry , optics , physics
The conformation of low‐salt LiDNA in fibrous form has been studied by Raman microspectroscopy. The recorded spectrum of a fibre soaked in ethanol is very similar to that of a fibre at low relative humidity. From x‐ray experiments it is known that in the latter case the DNA adopts the C conformation. Therefore, it can be concluded that this is also the case in a low‐salt LiDNA fibre soaked in ethanol. Further, it has been observed that the DNA conformation did not change when the excess ethanol was allowed to evaporate. The C form is also found when the fibre is in contact with ethanol vapour only. These results are in agreement with x‐ray studies. However, our experiments with a LiDNA fibre in tert ‐butanol are not in agreement with x‐ray results. Only when the tert ‐butanol is absolutely free from water is a state intermediate between the B and C forms found.