The effect of stacking on a low‐frequency vibrational band in an aqueous solution of adenylyl‐3′,5′‐cytidine (ApC)

Low‐frequency Raman spectra from 20 to 400 cm −1 of a 0.1 M aqueous solution of adenylyl‐3′,5′‐cytidine (ApC) ammonium salt were studied. An aqueous solution of ammonium chloride was used as reference. Experimental data were transformed to the R ( ν ) representation described previously. Spectra were obtained at temperatures between 0 and 70 °C. A broad band with a maximum below 100 cm −1 was assigned to water/base associate. This result corroborates previous assignments for nucleosides and nucleotides.