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In vivo studies of nucleic acid by ultraviolet resonance Raman spectroscopy on eucaryotic living cells
Author(s) -
Nocentini S.,
Chinsky L.
Publication year - 1983
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250140104
Subject(s) - nucleic acid , raman spectroscopy , resonance raman spectroscopy , resonance (particle physics) , chemistry , spectroscopy , absorption (acoustics) , photochemistry , in vivo , excited state , ultraviolet , fluorescence , analytical chemistry (journal) , biochemistry , materials science , organic chemistry , biology , optics , optoelectronics , atomic physics , physics , composite material , microbiology and biotechnology , quantum mechanics
We observe for the first time spectra of nucleic acids of living cells, obtained by resonance Raman spectroscopy excited in the u.v. range (257 nm). This wavelength which is close to that of the maximum of absorption of the main components of nucleic acids allows the resonance Raman effect to be obtained and the fluorescent contribution of aromatic amino acids present in proteins to be avoided.