Premium
Raman studies of L‐histidine and related compounds in aqueous solutions
Author(s) -
Tasumi Mitsuo,
Harada Issei,
Takamatsu Tadahisa,
Takahashi Seizo
Publication year - 1982
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250120212
Subject(s) - imidazole , raman spectroscopy , histidine , chemistry , aqueous solution , deuterium , ring (chemistry) , stereochemistry , crystallography , organic chemistry , physics , quantum mechanics , optics , enzyme
The Raman spectra of L‐histidine, L‐histidine‐ 13 C (labelled at C 2 of the imidazole ring) and related compounds, namely, imidazole, 4‐methylimidazole, N‐acetyl‐L‐histidine methylamide, were observed in H 2 O and D 2 O solutions at various pH and pD values. A strong Raman band at around 1410 cm −1 was found for these compounds in acidic D 2 O solutions, and it was assigned to a mode characteristic of the N‐deuterated imidazolium ring (Im + ) in which the N 1 C 2 N 3 symmetric stretching and ND bending vibrations are mixed. It is concluded that this band would be useful as a probe for studying the ionization states of histidine residues in proteins.