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Selective ultraviolet resonance Raman excitation of individual chromophores in nicotinamide adenine dinucleotide
Author(s) -
Rodgers Edward G.,
Peticolas Warner L.
Publication year - 1980
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250090606
Subject(s) - raman spectroscopy , nicotinamide adenine dinucleotide , chromophore , resonance (particle physics) , chemistry , ultraviolet , photochemistry , resonance raman spectroscopy , excitation , nicotinamide , analytical chemistry (journal) , nad+ kinase , materials science , enzyme , optics , biochemistry , optoelectronics , organic chemistry , atomic physics , physics , electrical engineering , engineering
Ultraviolet resonance Raman spectra (UVRRS) of reduced nicotinamide adenine dinucleotide (NADH) has been taken with ultraviolet laser excitation wavelengths (λ e ) of 273 nm, 330 nm and 351.1 nm. These Raman spectra have been compared with the resonance Raman spectra of the model compounds adenosine mononucleotide phosphate (AMP), with λ e = 300 nm, and with reduced methyl nicotinamide (MNH), with λ e = 351.1 nm, and with the classical Raman spectrum of NADH, with λ e = 514.5 nm. The UVRR spectrum of NADH with λ e = 273 nm resembles exclusively that of AMP while the UVRR spectra with λ e = 330 nm and λ e = 351.1 nm show no AMP bands but only those of MNH. The classical Raman spectrum of NADH is shown to be a superposition of bands in the resonance Raman spectra of the two chromophores but includes as well the sugar‐phosphate bands. Thus it is possible selectively to excite a UVRRS of either chromophore independently of the other. This information may prove helpful in developing techniques of UVRRS in studying NADH‐enzyme complexes since only very dilute (˜5×10 −4 M ) concentrations of NADH are needed for the resonant Raman spectra.