Premium
Fluorescence‐free resonance Raman spectra of reduced nicotinamide adenine dinucleotide via ultraviolet excitation
Author(s) -
Bowman W. David,
Spiro Thomas G.
Publication year - 1980
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250090605
Subject(s) - raman spectroscopy , chemistry , nicotinamide , nicotinamide adenine dinucleotide , resonance (particle physics) , raman scattering , fluorescence , excitation , ultraviolet , analytical chemistry (journal) , photochemistry , nad+ kinase , atomic physics , materials science , optics , enzyme , biochemistry , organic chemistry , optoelectronics , physics , electrical engineering , engineering
Excitation of reduced nicotinamide adenine dinucleotide (NADH) on the high‐energy side (333.6 nm) of its ultraviolet absorption band shifts the Raman scattering away from the fluorescence band and produces a good quality resonance Raman spectrum. It is dominated by the amide I (CO stretch) mode, at 1687 cm −1 , but several weaker bands are observed, and are tentatively assigned to amide II and III, and ring modes, consistent with N 2 H 2 and 4‐ 2 H isotope shifts. An identical spectrum is observed for nicotinamide mononucleotide (NMNH), indicating that the adenine and nicotinamide portions of NADH do not interact.