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A difference in polarizability between normal and sickle cell oxyhemoglobin as revealed by resonance Raman spectroscopy
Author(s) -
Barrett T. W.
Publication year - 1979
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250080211
Subject(s) - raman spectroscopy , polarizability , resonance raman spectroscopy , resonance (particle physics) , hemoglobin , nuclear magnetic resonance , dipole , chemistry , polarization (electrochemistry) , molecular physics , analytical chemistry (journal) , optics , atomic physics , molecule , physics , biochemistry , organic chemistry , chromatography
This report describes differences in the polarization of the resonance Raman lines at 1317, 1142 and 1012 cm −1 with respect to an exciting wavelength (5730 Å), which is within the long wavelength absorption region, for human normal hemoglobin AA versus sickle cell hemoglobin AS. The evidence indicates that the NC a band region differs in the two hemoglobins. These differences may be related to the differences in dipole moment between normal and sickle cell hemoglobin originally reported by Pauling. 1 The usefulness of the resonance Raman technique in providing additional microscopic information in a study of abnormal hemoglobins is discussed.