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Laser Raman spectroscopy of biomolecules. X‐frequency and intensity of the phosphodiester stretching vibrations of cyclic nucleotides
Author(s) -
Forrest G.,
Lord R. C.
Publication year - 1977
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250060108
Subject(s) - phosphodiester bond , raman spectroscopy , chemistry , nucleotide , polynucleotide , phosphate , spectroscopy , biomolecule , infrared spectroscopy , analytical chemistry (journal) , optics , organic chemistry , rna , biochemistry , physics , quantum mechanics , gene
Phosphate frequencies for eight nucleoside 3′,5′‐ and four nucleoside 2′, 3′‐cyclic monophosphates have been determined by Raman and infrared spectroscopy based on comparisons with modified cyclic nucleotides and model cyclic phosphates. To a first approximation the symmetric diester stretching frequency, ν s (OPO), is determined by the OPO bond angle. However, substitution on the phosphate ring also affects the frequency and intensity of ν s (OPO). These results are discussed in terms of the use of ν s (OPO) to determine the degree of backbone order in polynucleotides.