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Effect of Na + ion concentration on tRNA conformation in solution studied by laser Raman spectroscopy
Author(s) -
Dobek A.,
Patkowski A.,
Labuda D.,
Augustyniak J.
Publication year - 1975
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250030106
Subject(s) - raman spectroscopy , chemistry , transfer rna , aqueous solution , ion , ionic strength , ionic bonding , molecule , spectroscopy , analytical chemistry (journal) , sodium , crystallography , chromatography , rna , biochemistry , organic chemistry , physics , quantum mechanics , optics , gene
Raman spectra of baker's yeast tRNA in NaCl aqueous solutions were measured as a function of sodium ion concentration throughout the 0–1.0 M range. Strong variations in intensity of the various bands allowed observation of the ionic strength‐dependent conformational transitions of the tRNA. The experimental data obtained point to the existence of five distinct conformations of the tRNA molecules in the range of Na + concentration investigated.
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