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Hemoglobin resonance Raman excitation profiles with a tunable dye laser
Author(s) -
Strekas Thomas C.,
Spiro Thomas G.
Publication year - 1973
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1250010411
Subject(s) - raman spectroscopy , molar absorptivity , resonance (particle physics) , raman scattering , resonance raman spectroscopy , chemistry , excitation , laser , dye laser , absorption band , absorption (acoustics) , wavelength , coherent anti stokes raman spectroscopy , analytical chemistry (journal) , molecular physics , molecular electronic transition , x ray raman scattering , materials science , atomic physics , optics , optoelectronics , molecule , physics , organic chemistry , composite material , electrical engineering , engineering , chromatography
Resonance Raman spectra have been obtained for oxy hemoglobin within the α absorption band, corresponding to the first π – π* transition, using a tunable dye (Rhodamine 6 G) laser. The excitation profiles demonstrate resonance at the electronic origin (0–0), as well as at the vibronic side‐band (0–1) frequency, as predicted by theory. The wavelength dependence of the Raman intensity follows the molar absorptivity, rather than its square. It is suggested, however, that this form of the wavelength dependence may be an artifact of the transition from resonance to pre‐resonance scattering.