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Near‐infrared Fourier transform surface‐enhanced Raman scattering spectroscopy of 1,4‐benzodiazepine drugs employing gold films over nanospheres
Author(s) -
Trachta Gerd,
Schwarze Bernd,
Brehm Georg,
Schneider Siegfried,
Hennemann Matthias,
Clark Timothy
Publication year - 2004
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1154
Subject(s) - raman spectroscopy , chemistry , raman scattering , adsorption , analytical chemistry (journal) , infrared spectroscopy , density functional theory , infrared , molecule , spectral line , fourier transform infrared spectroscopy , moiety , computational chemistry , stereochemistry , chromatography , organic chemistry , optics , astronomy , physics
SERS spectra of eight benzodiazepine drugs were recorded with an FT‐NIR Raman spectrometer employing AuFONs (gold films over nanospheres) as SERS‐active substrates. A tentative assignment of the vibrational bands was made by comparison with the Raman spectra of crystalline reference samples and the results of density functional theory (DFT) calculations. It is concluded that the bands observed in the SERS spectra are almost exclusively due to vibrations located on rings a and b of the benzodiazepine moiety. The latter should be adsorbed with the molecular plane essentially orthogonal to the metal surface. However, different adsorption geometries are postulated for derivatives containing NH or NCH 3 groups. It is demonstrated that a few hundred nanograms of analyte per well are sufficient to record spectra that allow identification and discrimination of the drugs. Such amounts can be collected at the output of an HPLC instrument employed for separating these drugs from blood serum. Copyright © 2004 John Wiley & Sons, Ltd.