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In situ Raman spectra of an NAD + ‐modified silver electrode at various potentials
Author(s) -
Yang Haifeng,
Zhang Zongrang,
Shen Guoli,
Yu Ruqin
Publication year - 2004
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1122
Subject(s) - raman spectroscopy , nad+ kinase , chemistry , electrode , saturated calomel electrode , monolayer , analytical chemistry (journal) , molecule , desorption , surface enhanced raman spectroscopy , electrode potential , electrolyte , electrochemistry , raman scattering , reference electrode , adsorption , chromatography , organic chemistry , optics , biochemistry , physics , enzyme
At various potentials, the in situ confocal Raman technique was employed to detect SERS signals from a silver electrode surface modified with NAD + through a self‐assembled monolayer (SAM) method. The SERS bands of the NAD + molecule are much more sensitive to changes in the applied potential compared with experimental results reported previously. It is demonstrated in detail that the NAD + molecules undergo reorientation at or desorption from the silver electrode surface with a potential shift from −0.5 to 0.0 V vs a saturated calomel electrode based on a charge‐transfer mechanism for explanation of the SERS signals. The effect of buffer electrolytes on the results of the SERS experiment was also investigated. Copyright © 2004 John Wiley & Sons, Ltd.