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Detection of single lipid bilayers with coherent anti‐Stokes Raman scattering (CARS) microscopy
Author(s) -
Potma Eric O.,
Xie X. S.
Publication year - 2003
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1045
Subject(s) - raman scattering , lipid bilayer , raman spectroscopy , microscopy , membrane , chemistry , phospholipid , vesicle , resolution (logic) , optical microscope , analytical chemistry (journal) , microscope , materials science , optics , scanning electron microscope , physics , chromatography , composite material , biochemistry , artificial intelligence , computer science
We investigated vibrational imaging of phospholipid bilayers with CARS microscopy. Single lipid membranes of supported bilayers, giant unilamellar vesicles and intact erythrocyte membrane are detected with the strong resonant signal of the C–H stretching vibration. In addition, it is shown that the CARS signal field of the lipids near the glass/water interface is amplified through mixing with the back‐reflected non‐resonant CARS field of the glass coverslip. Furthermore, interference effects between two separate bilayers are observed, allowing intermembrane distances to be determined beyond the diffraction‐limited resolution of the microscope. Copyright © 2003 John Wiley & Sons, Ltd.