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Applicability of laser‐induced Raman microscopy for in situ monitoring of imine formation in a glass microfluidic chip
Author(s) -
Lee Moonkwon,
Lee JongPhil,
Rhee Hakjune,
Choo Jaebum,
Gyu Chai Young,
Kyu Lee Eun
Publication year - 2003
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/jrs.1038
Subject(s) - raman spectroscopy , raman microscope , analytical chemistry (journal) , laser , in situ , imine , microscopy , scanning electron microscope , chemistry , microfluidics , materials science , raman scattering , nanotechnology , optics , chromatography , organic chemistry , catalysis , composite material , physics
Laser‐induced Raman microscopy has been used to illustrate its applicability for the in situ monitoring of imine formation reaction in a glass microfluidic chip. In order to monitor the diffusion process in a micro channel, the Raman spectra were measured at various points along the channel with a constant flow rate of 2.7 µl min −1 . Time‐dependent Raman spectra were also measured without flow in order to monitor the variation of Raman peaks to a complete conversion. The disappearance of the CO stretching peak at 1700 cm −1 of the reactant, benzaldehyde, and the appearance of the Raman peak for the product, an imine, at 1628 cm −1 were successfully monitored. In addition, the intensity increases of three phenyl stretching modes in the 1550–1630 cm −1 region were also observed. The increase in Raman intensity for this vibrational mode is caused by an effective π‐electron conjugation between two phenyl rings through the —CN—bridging group of the product. Laser‐induced Raman microscopy enables us to monitor in situ product formation and to obtain detailed structural information in a glass microfluidic chip. Copyright © 2003 John Wiley & Sons, Ltd.