Premium
An effective antioxidant defense provides protection against zinc deficiency‐induced oxidative stress in Zn‐efficient maize plants
Author(s) -
Tewari Rajesh K.,
Kumar Praveen,
Sharma Parma Nand
Publication year - 2019
Publication title -
journal of plant nutrition and soil science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 87
eISSN - 1522-2624
pISSN - 1436-8730
DOI - 10.1002/jpln.201800622
Subject(s) - glutathione reductase , tbars , chemistry , antioxidant , lipid peroxidation , zinc , oxidative stress , glutathione , peroxidase , dry matter , superoxide dismutase , thiobarbituric acid , glutathione peroxidase , zinc deficiency (plant disorder) , food science , biochemistry , botany , enzyme , biology , organic chemistry
Maize plants ( Zea mays L. cv. Ganga 2 and cv. Jaunpuri satha) were grown in solution culture under glasshouse conditions at deficient (0 µM) and normal (1 µM) levels of Zn supply. Appearance of visible effects characteristic of Zn deficiency, depression in plant growth, and dry matter yield of the plants indicated that Ganga 2 was more susceptible to Zn deficiency than Jaunpuri satha. Higher susceptibility of Ganga 2 to Zn deficiency was also manifested by a greater decrease in plant dry mass and an increased accumulation of TBARS (thiobarbituric acid reactive substance, describing lipid peroxidation). While total SOD activity was decreased in Zn deficient plants of Ganga 2, it was increased marginally in case of Jaunpuri satha. The marginal increase in total SOD activity in the Zn‐deficient Jaunpuri satha plants was a result of a marked increase in non‐CuZn SOD and only a slight decrease in CuZn SOD. Though Zn deficiency increased H 2 O 2 concentration and the activities of H 2 O 2 ‐scavenging enzymes in both the cultivars, there was less increase in H 2 O 2 concentration and the activities of peroxidase, ascorbate peroxidase and glutathione reductase were more prominently increased in the Zn‐efficient Jaunpuri satha. Plants of the susceptible variety, Ganga 2, accumulated higher concentrations of glutathione disulfide. It is concluded that the significant decreases in the activities of CuZn SOD (CN‐sensitive SOD) and glutathione reductase, and high concentrations of H 2 O 2 predisposed Zn‐deficient Ganga 2 plants to more severe oxidative stress than those of Jaunpuri satha and, therefore, contributed to a greater decrease in dry matter production.