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Nitrogen fertilization and maturity influence the phenolic concentration of wheat grain ( Triticum aestivum )
Author(s) -
Stumpf Beate,
Yan Feng,
Honermeier Bernd
Publication year - 2015
Publication title -
journal of plant nutrition and soil science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 87
eISSN - 1522-2624
pISSN - 1436-8730
DOI - 10.1002/jpln.201400139
Subject(s) - chemistry , human fertilization , nitrogen , maturity (psychological) , caryopsis , agronomy , wheat grain , poaceae , biology , organic chemistry , psychology , developmental psychology
This work investigated the influence of N fertilization and grain maturity on total phenolic concentration (TPC) of wheat caryopses. A pot experiment was conducted, using soft spring wheat ( Triticum aestivum cv. Thasos) which was treated with four different amounts of nitrogen (0.25–2.00 g N pot −1 ) and harvested at three different development stages (medium milk stage, late milk stage, and dough maturity). Phenolic compounds were extracted and analyzed as total phenolic concentration in three discrete fractions: free soluble, conjugated soluble and insoluble bound forms. TPC of free phenolic compounds rose with increasing N supply while TPC of conjugated soluble phenolics decreased at the same time. Insoluble phenolics were less affected by N treatment. Total phenolic concentration also changed with the development stage of caryopses and reached a peak at the late milk stage.