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In vivo staining of reduced iron by 2,2′ bipyridine in rice exposed to iron toxicity
Author(s) -
Engel Katrin,
Asch Folkard,
Becker Mathias
Publication year - 2012
Publication title -
journal of plant nutrition and soil science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 87
eISSN - 1522-2624
pISSN - 1436-8730
DOI - 10.1002/jpln.201200096
Subject(s) - toxicity , in vivo , chemistry , staining , rice plant , genotype , 2,2' bipyridine , nuclear chemistry , food science , biochemistry , biology , horticulture , gene , microbiology and biotechnology , genetics , organic chemistry , crystal structure
Abstract A protocol for a novel method to visualize Fe(II) in rice tissues is proposed. The method is based on the selective formation of a purple‐red color complex of 2,2′ bipyridine and Fe(II). Rice genotypes were exposed to 18 mM Fe(II) in nutrient solution for 2 d. Root systems of intact plants were subsequently placed in 2,2′ bipyridine solutions. The formation of the [Fe(bipy)<$>_3^{2+}<$>] color complex was visualized using bifocal microscopy. The method may improve the selection of genotypes during breeding for Fe‐toxicity resistance of rice.