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Phosphorus‐31–nuclear magnetic–resonance spectroscopy to trace organic dung phosphorus in a temperate grassland soil
Author(s) -
Bol Roland,
Amelung Wulf,
Haumaier Ludwig
Publication year - 2006
Publication title -
journal of plant nutrition and soil science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 87
eISSN - 1522-2624
pISSN - 1436-8730
DOI - 10.1002/jpln.200521771
Subject(s) - chemistry , phosphorus , topsoil , grassland , soil water , nutrient , agronomy , soil test , subsoil , zoology , environmental chemistry , biology , ecology , organic chemistry
Cattle dung contributes to hot‐spot inputs of nutrients to grassland systems, but not much is known about its organic P (P o ) composition and fate in the grassland soils. We used 31 Phosphorus (P)–Nuclear Magnetic–Resonance (NMR) spectroscopy of alkaline soil extracts to examine potentials for tracing of different functional P o forms into a temperate grassland soil amended with dung. The proportion of monoester, DNA‐diester, and phospholipid+teichoic acid P were comparable in dung extracts, but the soil was dominated by monoester P. The temporal trends in the DNA‐diester P–to–monoester P (D DNA M) and diester P–to–monoester P (DM) ratio of dung, native soil, and soil amended with dung were monitored in the 70 d field experiment. The D DNA M and DM ratio in the dung‐amended soil (0–1 and 1–5 cm depth) were always intermediate between the dung and (unamended) control soil. Clearly, extracted soil P was a mixture of incorporated dung‐derived P and native soil P. The dung‐P contribution in the 0–1 cm samples peaked at 47% of the total extracted P at day 70 and at 15% after 42 d in the 1–5 cm soil depth (based on the DM ratio). The proportions of dung‐derived P and C in the soil were positively correlated with: 1) topsoil, using the D DNA M ratio (r 2 = 0.975), and 2) top‐ and subsoil, using the DM ratio (r 2 = 0.656). We concluded that our D DNA M and DM‐P ratios approach (obtained from solution‐ 31 P NMR) did trace successfully the short‐term dynamics and fate of dung P o in soil. It indicated that dung‐derived P o varied as rapidly in soil as the dung‐derived C.