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Adding dissolved organic carbon to simulate freeze‐thaw related N 2 O emissions from soil
Author(s) -
Sehy Ulrike,
Dyckmans Jens,
Ruser Reiner,
Munch Jean Charles
Publication year - 2004
Publication title -
journal of plant nutrition and soil science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 87
eISSN - 1522-2624
pISSN - 1436-8730
DOI - 10.1002/jpln.200421393
Subject(s) - dissolved organic carbon , chemistry , soil water , environmental chemistry , denitrification , nitrate , total organic carbon , nitrogen , soil science , environmental science , organic chemistry
It has been assumed that high winter N 2 O emissions from soils are the result of increased amounts of microbially available organic C liberated during freezing and metabolized during subsequent thawing. In a laboratory experiment, we attempted to simulate freeze‐thaw events by adding dissolved organic C (DOC) to sieved soil of high water content (95% water‐filled pore space). In a full factorial design, CO 2 and N 2 O emissions of a) soil samples provided with DOC extracted from frozen soil and b) soil samples frozen for 46 days and thawed were compared. Additionally, NO $_3^-$ , DOC and microbial ATP contents of all treatments were repeatedly analyzed during the experiment. The addition of DOC to unfrozen soil (–F+C) resulted in a substantial (22‐fold) increase in N 2 O emissions as compared to the control (–F–C). However, following thawing, the increase in N 2 O emissions was much larger (828‐fold in +F–C and 1243‐fold in +F+C). Freezing, but not the addition of DOC led to increased CO 2 emissions. Neither treatment affected microbial adenylate content. By adding 15 N‐labeled nitrate to the soil samples, the main process leading to elevated N 2 O flux rates after both DOC addition and freeze‐thaw treatment was identified as denitrification. We conclude that the availability of C substrate plays an important role for freeze‐thaw‐related N 2 O emissions. However, the fact that the simulated treatment and the freeze‐thaw treatment yielded significantly different amounts of N 2 O suggests that both quantity and quality of available C differed between the treatments. The localization of the liberated substrate, i.e. , the availability in situ , seems to be of major importance for the amount of N 2 O produced.