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The automated determination of glucosamine, galactosamine, muramic acid, and mannosamine in soil and root hydrolysates by HPLC
Author(s) -
Appuhn Astrid,
Joergensen Rainer Georg,
Raubuch Markus,
Scheller Edwin,
Wilke Brigitte
Publication year - 2004
Publication title -
journal of plant nutrition and soil science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 87
eISSN - 1522-2624
pISSN - 1436-8730
DOI - 10.1002/jpln.200321302
Subject(s) - muramic acid , hydrolysate , chemistry , derivatization , chromatography , galactosamine , amino sugar , glucosamine , reagent , high performance liquid chromatography , hydrolysis , biochemistry , peptidoglycan , organic chemistry , enzyme
Amino sugars make a significant contribution to soil organic N and are mainly of microbial origin. The most important amino sugars in soil are glucosamine, galactosamine, muramic acid, and mannosamine. A method was developed for the simultaneous determination of these four amino sugars by high‐performance reverse‐phase liquid chromatography in standard solutions, soil and root hydrolysates. Pre‐column derivatization with o‐phthaldialdehyde (OPA) was used in an automated sample injector with thermostatic regulation of the reagent at 4 °C. The separation of the four amino sugars was fully satisfactory and was not disturbed by other fluorescent components in the soil and root hydrolysates.