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Tandem size exclusion chromatography‐Polyacrylamide gel electrophoresis of humic acids
Author(s) -
Trubetskoj Oleg A.,
Trubetskaya Olga E.,
Afanasieva Gaida V.,
Reznikova Olga I.,
Hermosin Bernardo,
SaizJimenez Cesareo
Publication year - 1998
Publication title -
zeitschrift für pflanzenernährung und bodenkunde
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 87
eISSN - 1522-2624
pISSN - 0044-3263
DOI - 10.1002/jpln.1998.3581610604
Subject(s) - chromatography , size exclusion chromatography , polyacrylamide , polyacrylamide gel electrophoresis , chemistry , tandem , gel permeation chromatography , biochemistry , materials science , organic chemistry , polymer chemistry , polymer , enzyme , composite material
Humic acids (HAs) from four soils were fractionated by size exclusion chromatography (SEC) on Sephadex G‐75. Three fractions were obtained in all humic acids, collected and assayed by Polyacrylamide gel electrophoresis (PAGE). The unfractionated HA from each soil was used as reference. Each chromatographic fraction formed one electrophoretic zone corresponding closely to one band of the reference sample with some admixture of the fractions preceding or following. The results indicate that fractionation of HAs by tandem SEC‐PAGE can be successfully used for obtaining fractions of reduced polydispersity and different electrophoretic mobilities. Pyrolysis/methylation‐gas chromatography‐mass spectrometry of the full size preparations of HA and fractions with exactly defined molecular size and electrophoretic mobility showed a different distribution in humic components, mainly lipids, lignin derivatives and N‐containing compounds.