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Basic strategies of molecular biological research in plant nutrition ‐ a review
Author(s) -
ArnholdtSchmitt Birgit
Publication year - 1996
Publication title -
zeitschrift für pflanzenernährung und bodenkunde
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 87
eISSN - 1522-2624
pISSN - 0044-3263
DOI - 10.1002/jpln.1996.3581590402
Subject(s) - biology , computational biology , gene , complementary dna , reporter gene , yeast , identification (biology) , genetically modified crops , transgene , microbiology and biotechnology , gene expression , genetics , botany
A review is given on basic methodical strategies of molecular biological research in plant nutrition. Membrane transport protein identification and analysis by the use of cDNA libraries and functional expression in yeast mutants and Xenopus oocytes is demonstrated. The various methods of gene isolation and analysis, including molecular data of model plants, are pointed out. The significance of research on transgenic plants permitting overexpression of introduced genes, antisense technique and analysis of promoter/reporter gene constructs is emphasized. The numerous examples of recent research simultanously indicate contemporary and future topics for molecular biological research in plant nutrition.