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Investigation on Soil Lipid Extraction with Different Organic Solvents
Author(s) -
Wu Quinglan,
Schleuss Uwe,
Blume HansPeter
Publication year - 1995
Publication title -
zeitschrift für pflanzenernährung und bodenkunde
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 87
eISSN - 1522-2624
pISSN - 0044-3263
DOI - 10.1002/jpln.19951580406
Subject(s) - chemistry , toluene , ethanol , benzene , thin layer chromatography , extraction (chemistry) , solvent , chromatography , gas chromatography , accelerated solvent extraction , organic solvent , nuclear chemistry , organic chemistry , chemical engineering , engineering
Soil lipid content is an important characteristic of the soil organic matter. One of the commonly used extractants for the determination of soil lipid is benzene/ethanol 1:1(v/v). In this study the toxic solvent mixture benzene/ethanol was substituted with the less harmful one toluene/ethanol 1:2.3 (v/v). Both solvent mixtures give a comparably high recovery of soil lipid and the composition of both lipid fractions are very similar according to their thin layer chromatography (TLC). Further investigations with NMR‐spectroscopy confirmed that the extracted lipid fraction consists mainly (7gt;90%) of structure elements of aliphatic hydrocarbons.