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Aluminium‐induced callose formation in root tips of Norway spruce (Picea abies (L.) Karst.)
Author(s) -
Jorns Axel C.,
HechtBuchholz Charlotte,
Wissemeier Alexander H.
Publication year - 1991
Publication title -
zeitschrift für pflanzenernährung und bodenkunde
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 87
eISSN - 1522-2624
pISSN - 0044-3263
DOI - 10.1002/jpln.19911540507
Subject(s) - callose , picea abies , ultrastructure , botany , nutrient , chemistry , staining , aluminium , cell wall , biology , biophysics , genetics , organic chemistry
The objective of this study was to examine whether aluminium (Al) induces callose formation in roots of Norway spruce as it does in soybean. Spruce seedlings were grown in Al‐free nutrient solution under controlled conditions in a growth chamber at pH 3.8. After 21 days 170 μM Al was added or not (controls) to the complete nutrient solution (molar Ca/Al ratio: 0.75). Callose could be detected in outer root‐tip cells of Al‐treated plants within 3 h, using fluorescence microscopy after staining with aniline blue. Prolonged Al treatment up to 24 h increased both the density of the callose deposits and the number of affected cell layers. Control plants showed no comparable callose deposits. Ultrastructural examinations showed cell‐wall appositions in Al‐treated root cells but not in controls. The possible implications of Al‐induced callose formation for nutrient and water uptake by roots are discussed.