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Reversed‐Phase High‐Performance Liquid Chromatography of naturally occurring phenolic acids in soils
Author(s) -
Kögel Ingrid
Publication year - 1983
Publication title -
zeitschrift für pflanzenernährung und bodenkunde
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 87
eISSN - 1522-2624
pISSN - 0044-3263
DOI - 10.1002/jpln.19831460413
Subject(s) - chromatography , high performance liquid chromatography , chemistry , resolution (logic) , reversed phase chromatography , gradient elution , elution , hydrophilic interaction chromatography , soil water , phase (matter) , reproducibility , chromatography column , organic chemistry , geology , artificial intelligence , computer science , soil science
A method for the analysis of phenolic acids in plant and soil materials by high‐performance liquid chromatography (HPLC) is described. Separation is by reversed‐phase chromatography, based on differential polarity of the investigated compounds. Optimal separation conditions are achieved by using a concave gradient elution. The HPLC technique offers excellent resolution, reproducibility and an analysis time of 30 Min.