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Effect of clay minerals on the activity of polysaccharide cleaving soil enzymes
Author(s) -
Pflug Werner
Publication year - 1982
Publication title -
zeitschrift für pflanzenernährung und bodenkunde
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 87
eISSN - 1522-2624
pISSN - 0044-3263
DOI - 10.1002/jpln.19821450509
Subject(s) - palygorskite , montmorillonite , kaolinite , chemistry , starch , cellulase , polysaccharide , enzyme , cellulose , amylase , clay minerals , sorption , biochemistry , nuclear chemistry , organic chemistry , adsorption , mineralogy
Kinetic analyses of the influence of montmorillonite, palygorskite and kaolinite on the activity of three polysaccharide cleaving soil enzymes revealed an inhibitory effect that is rather due to the structure than to chemical characteristics of the clays. The fibrous palygorskite was found to be highly effective towards the starch cleaving α‐amylase and amyloglucosidase, whereas the layer silicates caused only a slight inhibition. Interactions with cellulase were affected by sorption of cellulose by montmorillonite and kaolinite. When the enzyme was allowed to be sorbed by the clays before cellulose was added, a clay‐enzyme complex is formed, which in contrast to the starch cleaving enzymes remains active. Compared to the native enzyme, the bound enzyme is not stabilized with respect to temperature, but the optimum pH is found to be at pH 6 instead of pH 4.8.