Premium
Antibiofilm effect of ozonized physiological saline solution on peri‐implant–related biofilm
Author(s) -
To Caroline C.,
Panariello Beatriz H. D.,
Spolidorio Denise M. P.,
Gossweiler Ana G.,
Duarte Simone
Publication year - 2021
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1002/jper.20-0333
Subject(s) - biofilm , saline , porphyromonas gingivalis , fusobacterium nucleatum , peri implantitis , chlorhexidine , streptococcus oralis , prevotella intermedia , chemistry , microbiology and biotechnology , antiseptic , aggregatibacter actinomycetemcomitans , anaerobic exercise , implant , dentistry , bacteria , periodontitis , medicine , biology , surgery , physiology , genetics , organic chemistry
Abstract Background Removal of dental plaque and local application of local chemical adjuncts, such as chlorhexidine (CHX), have been used to control and treat peri‐implant disease. However, these methods can damage the surface properties of the implants or promote bacterial resistance. The application of ozone as an adjunctive treatment represents a new approach in the management of peri‐implantitis. Thus, the purpose of this study was to evaluate the antimicrobial effect of ozonized physiological saline solution in different concentrations against oral biofilms developed on titanium surface. Methods Single and multi‐species biofilms of Porphyromonas gingivalis , Fusobacterium nucleatum , and Streptococcus oralis were formed on titanium specimens for 5 days in anaerobic conditions. Biofilms were treated with ozonized saline solution at different concentrations (25, 50, and 80 μg/NmL), for 30 seconds and 1 minute. CHX (0.12%) and saline solution (0.89% NaCl) were used as positive and negative controls, respectively. Bacterial viability was quantified by colony forming units (CFU mL −1 ), and biofilm images were acquired by confocal laser scanning microscopy (CLSM). Data were analyzed by parametric test (ANOVA) with Tukey post‐hoc test ( P < 0.05). Results Ozonized saline solution showed antibiofilm activity at a concentration of 80 μg/NmL for 30 seconds and 1 minute, reducing, mainly, Porphyromonas gingivalis viability, with 2.78 and 1.7 log 10 CFU mL −1 of reduction in both single and multi‐species biofilms, respectively, when compared to the control (saline), whereas CHX reduced 1.4 and 1.2 log 10 CFU mL −1 . Conclusion Ozonized saline solution has antibiofilm activity, with better effect when applied for 1 minute at 80 μg/NmL, being a promising candidate therapy for the treatment of peri‐implant diseases.