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2,3,5,4′‐Tetrahydroxystilbene‐2‐O‐β‐ d ‐glucoside promotes the effects of dental pulp stem cells on rebuilding periodontal tissues in experimental periodontal defects
Author(s) -
Lin ChiYu,
Tsai MinShi,
Kuo PoJan,
Chin YuTang,
Weng ITsen,
Wu Yen,
Huang HawMing,
Hsiung ChaoNan,
Lin HungYun,
Lee ShengYang
Publication year - 2021
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1002/jper.19-0563
Subject(s) - dental pulp stem cells , molar , dentistry , proliferating cell nuclear antigen , osteopontin , vascular endothelial growth factor , angiogenesis , periodontitis , pulp (tooth) , periodontology , medicine , chemistry , immunohistochemistry , pathology , mesenchymal stem cell , vegf receptors , cancer research
Background This study aimed to investigate the regenerative effects of 2,3,5,4′‐tetrahydroxystilbene‐2‐O‐β‐ d ‐glucoside (THSG)‐treated human dental pulp stem cells (DPSC) on the healing of experimental periodontal defects in rats. Methods The maxillary first molars of 30 male Sprague–Dawley rats were extracted, and after healing, bilateral periodontal defects were surgically created mesially in second molars. The defects were treated with Matrigel (as control), DPSC, or DPSC + THSG. After 2 weeks, the healed defects were evaluated using microcomputed tomography and through histological and immunohistochemical analyses. Results In the microcomputed tomography analysis, more new bone formation in the DPSC and DPSC + THSG groups was observed compared with the control group. The periodontal bone supporting ratio in site with DPSC + THSG was significantly higher than that in DPSC. Histologically, an enhanced new bone formation and more significant periodontal attachment were observed in the DPSC + THSG group. The expression levels of proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGF), and osteopontin (OPN) in the DPSC + THSG group were significantly greater than those in other groups. Conclusions THSG‐revolutionized DPSCs significantly shortened the regenerative period of periodontal defects by enhancing the cell recruitment and possibly the angiogenesis in rat models, which illustrate the critical implications for a clinical application and provide a novel tactic for periodontitis treatment.