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Effect of non‐surgical periodontal treatment on gingival crevicular fluid hypoxia inducible factor‐1 alpha, vascular endothelial growth factor and tumor necrosis factor‐alpha levels in generalized aggressive periodontitis patients
Author(s) -
Afacan Beral,
Keleş Yücel Zeynep Pinar,
Paşali Çiğdem,
Atmaca İlhan Harika,
Köse Timur,
Emingil Gülnur
Publication year - 2020
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1002/jper.19-0521
Subject(s) - medicine , vascular endothelial growth factor , periodontitis , tumor necrosis factor alpha , gastroenterology , scaling and root planing , bleeding on probing , hypoxia (environmental) , cytokine , clinical significance , biomarker , aggressive periodontitis , chronic periodontitis , vegf receptors , biochemistry , chemistry , organic chemistry , oxygen
Background Hypoxia‐inducible angiogenic pathway involving hypoxia inducible factor‐1 alpha (HIF‐1α), vascular endothelial growth factor (VEGF) and tumor necrosis factor‐alpha (TNF‐α) may regulate several biological processes related to inflammation. The present study aimed to assess the effect of non‐surgical periodontal treatment on gingival crevicular fluid (GCF) HIF‐1α, VEGF, and TNF‐α levels in generalized aggressive periodontitis (G‐AgP). Methods Twenty G‐AgP patients and 20 periodontally healthy individuals were included. G‐AgP patients received scaling and root planning (SRP), per quadrant at a 1‐week‐interval, performed with ultrasonic and periodontal hand instruments. GCF samples were collected and clinical periodontal parameters including probing depth, clinical attachment level, gingival index and plaque index were recorded at baseline, 1 and 3 months after treatment. Biomarker levels in GCF were analyzed by ELISA. Results At baseline all clinical parameters and GCF HIF‐1α, VEGF, and TNF‐α levels were significantly higher in G‐AgP patients compared to healthy control ( P < 0.05). All clinical parameters improved over the 3‐month‐period in G‐AgP patients ( P < 0.05). GCF HIF‐1α levels in G‐AgP reduced at 1 and 3 months post‐treatment, however, this did not reach to statistical significance ( P > 0.05). GCF VEGF and TNF‐α levels remained unchanged throughout the study period ( P > 0.05). Conclusions Within the limitations of the present study, although HIF‐1α seems to possess a potential diagnostic value for G‐AgP, it might not be a proper predictor of clinically favorable treatment outcome. SRP plus different adjunctive therapies could provide better information about the prognostic role of hypoxia‐inducible angiogenic pathway in G‐AgP.