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Accelerated degradation of collagen membranes in type 1 diabetic rats is associated with increased expression and production of several inflammatory molecules
Author(s) -
Zoabi Hasan,
Nemcovsky Carlos E.,
Bender Omer,
Moses Ofer,
Weinreb Miron
Publication year - 2020
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1002/jper.19-0503
Subject(s) - tumor necrosis factor alpha , inflammation , matrix metalloproteinase , blot , peripheral blood mononuclear cell , chemistry , streptozotocin , membrane , proinflammatory cytokine , messenger rna , endocrinology , medicine , diabetes mellitus , biochemistry , in vitro , gene
Background Membrane durability is critical for regenerative procedures. We reported previously that type 1‐like diabetes in rats accelerates the degradation of collagen membranes and we tested here whether this is associated with increased local production of inflammatory molecules as part of a diabetes‐induced chronic inflammation around and within the membranes. Methods Collagen membrane discs were implanted under the scalp in diabetic (streptozotocin‐induced) and control rats, which were sacrificed after 2 or 3 weeks. Total RNA and proteins were isolated from the membrane and its surrounding tissues and the expression and production of six inflammatory molecules (interleukin‐6 [IL‐6], tumor necrosis factor alpha [TNFα], matrix metalloproteinase [MMP]‐9, macrophage migration inhibitory factor [MIF], MIP‐1α, and MIP‐2α) was measured using real‐time PCR and western blotting, respectively. Minimal histological analysis of the membranes was conducted to conform to previous studies. Results Hyperglycemia resulted in reduced membrane thickness (by 10% to 25%) and increased mononuclear infiltrate inside the membrane. mRNA and protein levels of IL‐6, TNFα, and MMP‐9 were elevated in diabetic rats both 2 and 3 weeks post‐surgery. The levels (both mRNA and protein) of MIF were increased at 2 weeks post‐surgery and those of MIP‐1α and MIP‐2α at 3 weeks. There was a very good match in the temporal changes of all examined genes between the mRNA and protein levels. Conclusions Elevated local production of inflammatory cytokines and MMPs, together with apparent mononuclear infiltrate and increased collagenolysis confirm that hyperglycemia leads to a chronic inflammation in and around the implanted collagen membranes, which reduces membrane longevity.