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A new target to ameliorate the damage of periodontal disease: The role of transient receptor potential vanilloid type‐1 in contrast to that of specific cannabinoid receptors in rats
Author(s) -
Ossola César A.,
Balcarcel Noelia B.,
Astrauskas Julia I.,
Bozzini Clarisa,
Elverdin Juan C.,
FernándezSolari Javier
Publication year - 2019
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1002/jper.18-0766
Subject(s) - am251 , trpv1 , pharmacology , medicine , cannabinoid , porphyromonas gingivalis , endocrinology , receptor , inflammation , periodontitis , cannabinoid receptor , chemistry , antagonist , transient receptor potential channel
Background Transient receptor potential vanilloid type‐1 (TRPV1) is expressed in oral tissues cells and its activity can be regulated by inflammation products and anandamide. The aim of the present study was to evaluate the effects of blocking TRPV1 or specific cannabinoid receptors 1 (CB1r) and 2 (CB2r) on periodontal status of rats subjected to experimental periodontitis (EP). Methods Male rats were distributed in groups 1) control, 2) lipopolysaccharide‐induced EP (LPS), and 3) LPS plus capsazepine (Capz, TRPV1 antagonist) application (LPS+Capz). EP was induced by injections of LPS (1 mg/mL) around first molars and treatment was performed with Capz (2 µg/mL) applied locally during 6 weeks. Additional experiment was performed by applying CB1r and CB2r antagonists (AM251 and AM630) to rats with EP. Results Capz prevented alveolar bone loss (ABL) on the external crests and in the interradicular bone of the first molars (periodontal space height: LPS, 270.7 ± 33.5µm versus LPS+Capz, 216.4 ± 19.9 µm; P <0.01). Inflammatory mediators, like tumor necrosis factor‐alpha and prostaglandin E 2 , increased by LPS‐induced EP, were diminished in gingival tissue of rats treated with Capz. In contrast, application of AM251 and AM630 exacerbated ABL and gingival inflammatory mediators, increased by LPS, altering also biomechanical properties. Conclusions TRPV1 blockade attenuates periodontal impairment in EP rats, since it reduces local inflammation, unlike CB1r and CB2r blockade. This work lays the foundation for developing therapeutics in humans based on the pharmacological manipulation of these receptors to treat periodontal disease.